Pre-embedding immunoelectron microscopy by peroxidase-labeled antibody method - Applications to endocrinology.
نویسندگان
چکیده
منابع مشابه
Post-embedding immunoelectron microscopy of tissue processed by rapid freezing and freeze-substitution without chemical fixatives.
With great interest we read the recent article by Usuda et al. (1), which describes the immunoelectron microscopy of tissue processed by rapid freezing and freeze-substitution without chemical fixatives. In their article, Usuda et al. say that “our result . . . should (sic) be the first demonstration of immunostaining of rapidly frozen tissue processed without chemical fixation.” We would like ...
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Sample preparation for transmission immunoelectron microscopy techniques is pivotal to preserve cell architecture and to maintain antigen epitopes. We propose a “non-classical” method in order to obtain the above-mentioned conditions. In a pilot series of experiments, we found a mixture of 2.0% paraformaldehyde/0.1% glutaraldehyde in 0.1 M PBS (pH 7.4) to be an optimal fixing solution. In this ...
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The ultrastructural detection and identification of human retroviruses--HTLV (human T-cell lymphotropic virus) and HIV (human immunodeficiency virus)--have become an everyday task for pathologists and virologists as well as for cell and molecular biologists. The development of better and conventionally available immunocytochemical techniques, such as pre- or postembedding immunocytochemical met...
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The direct action of 17 p-estradiol and cholesterol on cells of the pituitary pars distalis, as revealed by staining with peroxidase-labeled antibody, was studied in female rats. Pellets of pure cholesterol and of estradiol mixed with cholesterol were implanted into the left lobe of the pars distalis 14-32 days after ovariectomy and left in place for 7-16 days. Rabbit antisera to rat prolactin,...
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Cytoplasmic immunoglobulin G (IgG) in normal human epidermis was defined by a peroxidase-labeled antibody method. A correlation between cytoplasmic staining and the serum level of IgG was found. Epidermal cells containing IgG were not present when the serum level of IgG was less than 1000 microgram/ml.
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ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 1981
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.14.383